DOI: 10.11607/jomi.1085218. Mar 2024,Language: EnglishFeng, Xi / Wang, Haicheng / Weng, Yuteng / Chen, Yongliang / Huang, Jie / Wang, Zuolin
Purpose: To explore the contribution of paired-related homeobox 1-positive cells to the
implant-induced osseointegration process in adult alveolar bone and the potential underlying
mechanisms. Materials and Methods: Cre recombinase-induced lineage tracing and cell
ablation were conducted in a murine dental implant model. Scratch and transwell assays were
used to assess MC3T3-E1 cell migration after paired-related homeobox 1 overexpression.
Single-cell RNA sequencing were applied to identify potential genes involved in pairedrelated
homeobox 1-positive cells-driven osteogenesis. Results: Paired-related homeobox 1-
positive cells were observed to accumulate in the peri-implant area in a time-dependent
manner. The number of these cells were found to reach its maximum on day 14.
Osseointegration in mice were noticeably impaired after ablation of paired-related homeobox
1-positive cells. Further, it was discovered that paired-related homeobox 1 promotes MC3T3-
E1 cell migration, a process which is indispensable for sound healing of peri-implant tissue.
Finally, Semaphorin 3C was detected exclusively and abundantly expressed by paired-related
homeobox 1-positive cells. Knockdown of semaphorin 3C in paired-related homeobox 1-
positive cells significantly weakened their osteogenic potential. Conclusion: Our data suggest
that paired-related homeobox 1-positive cells contribute to the osseointegration process under
stress stimulation and semaphorin 3C may play a critical role in paired-related homeobox 1-
positive cell-driven osteogenesis. Paired-related homeobox 1 could significantly promote
MC3T3-E1 cell migration.
Keywords: paired-related homeobox 1-positive cells, osseointegration, alveolar bone, semaphorin 3C
